Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012040_07d3 GTGAATTCGAAAGTAACCCCTTTAGGAGATAG(+ACTCAC)GTACCAGGAAAAGCCCCTCCCTCAACAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605012038_re1f AAGCCATTTTGTTGAGGGAGGGGCTTTTCCTG(+ACTCAC)GTACCTATCTCCTAAAGGGGTTACTTTCGAA NGG 10 8 17 8 24 0 100 5 10
    240605012037_i52j CAAGGCCCTGAAATCTAACCTTATCCACTAGG(+ACTCAC)GTACCTTCCTCAGCAGTGTTTCCTTCAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605012035_gqai ACCAAACTCTGAAGGAAACACTGCTGAGGAAG(+ACTCAC)GTACCCTAGTGGATAAGGTTAGATTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605012034_hqhy TTCAGAGAACAGCCCCCTCACTGGCTTTTGAG(+ACTCAC)GTACCTTTTATGAGGACATTAGGACTGATAG NGG 10 8 17 8 24 0 100 5 10
    240605012033_mef0 CTTTTCTATCAGTCCTAATGTCCTCATAAAAG(+ACTCAC)GTACCTCAAAAGCCAGTGAGGGGGCTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605012031_fw3s TACAGAGCACACACAAAATCAAAGATCCAAAG(+ACTCAC)GTACCAATCTTCCGTGGAAAACCCAAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605012029_jdwl AGTTTGGGGTTTGGGTTTTCCACGGAAGATTG(+ACTCAC)GTACCTTTGGATCTTTGATTTTGTGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605012028_khbo AGTGTGTGATCAGTGAACTGTCATTTCTTCAG(+ACTCAC)GATCCACTTCACTTGTCTTCTGCACAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605012027_84nu CTATCTGGCCTGTGCAGAAGACAAGTGAAGTG(+ACTCAC)GATCCTGAAGAAATGACAGTTCACTGATCAC NGG 10 8 17 8 24 0 100 5 10