Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011955_a4rc ACAGCTCCTGTGTCCTTGTCTCTAGCAGGGCG(+ACTCAC)GTACCTGTCAGCAGCCAAGGGCAAGGCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605011953_5ojr TTCCAGCAAGCCTTGCCCTTGGCTGCTGACAG(+ACTCAC)GTACCGCCCTGCTAGAGACAAGGACACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605011952_jszy GCTTAAGTATAGATAACCCTATATCAGGGGAG(+ACTCAC)GTACCGTGTTCTAATGCTGCAAGTGCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605011950_6ie3 GATAGCCCAAGCACTTGCAGCATTAGAACACG(+ACTCAC)GTACCTCCCCTGATATAGGGTTATCTATACT NGG 10 8 17 8 24 0 100 5 10
    240605011947_x2i5 CCACATGGGGTGAGTCATGACTAGCTCCTGGG(+ACTCAC)GATCCGACACCCTCTTCTAGTCTCCAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605011946_a1u3 CGAGTGCCCTTGGAGACTAGAAGAGGGTGTCG(+ACTCAC)GATCCCCAGGAGCTAGTCATGACTCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240605011945_owtv CACGGATGCCCATGTTTGCCTAGAGTCACTAG(+ACTCAC)GATCCCCACATCTCTCCACGCAGTGTATCTA NGG 10 8 17 8 24 0 100 5 10
    240605011943_kltp GCAGCTAGATACACTGCGTGGAGAGATGTGGG(+ACTCAC)GATCCTAGTGACTCTAGGCAAACATGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605011942_salk GGTTGATTGGCGCTCAAGTCCAGGGTGTGTGG(+ACTCAC)GTACCTGTGGCAAAGTAAGGCAGAAGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605011940_cm1u GTTTATCCAACTTCTGCCTTACTTTGCCACAG(+ACTCAC)GTACCCACACACCCTGGACTTGAGCGCCAAT NGG 10 8 17 8 24 0 100 5 10