Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011922_mfom GTCCTGGAAAGTACAAAAACATCTACAAGCGG(+ACTCAC)GATCCCGGTGTACCTGGAAGTGCAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605011920_ato9 TGAGGACAGGGCTGCACTTCCAGGTACACCGG(+ACTCAC)GATCCCGCTTGTAGATGTTTTTGTACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605011919_mffw TCTAACTCAAGCCAGACCCCCTACAACAAGAG(+ACTCAC)GATCCAGGGCCAGCCATGGTAGAGTCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605011917_9cet GAAAACCTTTGACTCTACCATGGCTGGCCCTG(+ACTCAC)GATCCTCTTGTTGTAGGGGGTCTGGCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605011915_75i7 GCACAGCTGCCAAGGCTTAGAAGGAGAAGCTG(+ACTCAC)GTACCGGCCTCTTCTTCAAAAGCAGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605011913_xxjj CAGCACCCTGGCTGCTTTTGAAGAAGAGGCCG(+ACTCAC)GTACCAGCTTCTCCTTCTAAGCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605011912_ar3d GACATTTGACAAGTGTCCAATTTCTCCAAAGG(+ACTCAC)GTACCTTAAGATTCTCAAAAAAAAAGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605011910_7usu TTTGAATTTACTTTTTTTTTGAGAATCTTAAG(+ACTCAC)GTACCCTTTGGAGAAATTGGACACTTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605011909_bvo9 ATTAATGTGGTTAAATCCAAAGTCTGAGGTAG(+ACTCAC)GTACCTGAGCCTTGTTATTTTGGAAATTCAA NGG 10 8 17 8 24 0 100 5 10
    240605011908_z2tb TGAATTTGAATTTCCAAAATAACAAGGCTCAG(+ACTCAC)GTACCTACCTCAGACTTTGGATTTAACCACA NGG 10 8 17 8 24 0 100 5 10