Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011841_lmt5 AGGGTGGCCTCAGCAGGTGGGAAAGAGGAAAG(+ACTCAC)GTACCAGATCAGGCTGAGGATTTGACATCAC NGG 10 8 17 8 24 0 100 5 10
    240605011840_dawm AATGATGGGTACATGAGACTTGATTACAGATG(+ACTCAC)GATCCCTTAACTCTGTGTTCATGTGATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605011838_b4ib GTACACAGAATCACATGAACACAGAGTTAAGG(+ACTCAC)GATCCATCTGTAATCAAGTCTCATGTACCCA NGG 10 8 17 8 24 0 100 5 10
    240605011837_m11n CAGGCCTGACAAGCGGTAGAATCACCAGCTAG(+ACTCAC)GTACCCAATCCACAATGAGGAAACCTTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605011835_hgmq TAGGATACAAAGGTTTCCTCATTGTGGATTGG(+ACTCAC)GTACCTAGCTGGTGATTCTACCGCTTGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605011833_7skf AACAGAAACTTTTATATTGTGCTGCTGCCTTG(+ACTCAC)GTACCACCCTTCAGTCTGTTCAAGAAACACC NGG 10 8 17 8 24 0 100 5 10
    240605011831_yiqt GTCTTGGTGTTTCTTGAACAGACTGAAGGGTG(+ACTCAC)GTACCAAGGCAGCAGCACAATATAAAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605011828_vec9 TGGTCAAATCACAGGTGCTTATGCAGCCCTGG(+ACTCAC)GATCCTTCCCGTGTGAACCCTGTGGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605011827_zduy GCACATGTGCTCCACAGGGTTCACACGGGAAG(+ACTCAC)GATCCCAGGGCTGCATAAGCACCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240605011825_vvfo ACTGTCTCCAGACACACCAGAAGAGGGCATCG(+ACTCAC)GATCCCATTACAGAATGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10