Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011807_77dh AAGAGGGAATTAGGAAATGAGGGAATCCATTG(+ACTCAC)GATCCTGGATTCTAGACTGAGACTCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605011805_ownx CATACCACACAGAGTCTCAGTCTAGAATCCAG(+ACTCAC)GATCCAATGGATTCCCTCATTTCCTAATTCC NGG 10 8 17 8 24 0 100 5 10
    240605011803_yk65 CATGAGGGAACCAGGCTAGGTGTTTAGCCTTG(+ACTCAC)GATCCCTGATCAAGTTCCATATCAGCTACCC NGG 10 8 17 8 24 0 100 5 10
    240605011801_6g5a CTTGGGGGTAGCTGATATGGAACTTGATCAGG(+ACTCAC)GATCCAAGGCTAAACACCTAGCCTGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605011759_n0t9 GTAGCTCTCAGTGGGACCTGTGGCTGTCCATG(+ACTCAC)GTACCCATGGCAATTGGCCCAGCCTGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605011756_8c7q CTGACATCCACAGGCTGGGCCAATTGCCATGG(+ACTCAC)GTACCATGGACAGCCACAGGTCCCACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605011755_zbzv TGTACGCGCGGCACAAAGGTCTGCACTCAGTG(+ACTCAC)GTACCTGCGGGTTGCCCAGCAGGGTTCACAC NGG 10 8 17 8 24 0 100 5 10
    240605011753_9t4j CCCGTGTGTGAACCCTGCTGGGCAACCCGCAG(+ACTCAC)GTACCACTGAGTGCAGACCTTTGTGCCGCGC NGG 10 8 17 8 24 0 100 5 10
    240605011752_ot6p CGCACCCGCTCCCGCGTGGTGGCTCTTAGCGG(+ACTCAC)GTACCCTGGGAGCGCCTGCCTCACGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605011749_oedx AGAAGGGCTCACGTGAGGCAGGCGCTCCCAGG(+ACTCAC)GTACCCGCTAAGAGCCACCACGCGGGAGCGG NGG 10 8 17 8 24 0 100 5 10