Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011700_vm4u ATGGACCAAGGGTTTCATGGGGCAGAAGGAGG(+ACTCAC)GTACCCAGAATTCCCAGCCCTCATCTGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605011658_q5qk GGCTGGGACCAGATGAGGGCTGGGAATTCTGG(+ACTCAC)GTACCCTCCTTCTGCCCCATGAAACCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240605011657_rn39 CCATTGCTTCCCCTTATAAGATGTCCCTGCAG(+ACTCAC)GTACCAGAGCCAGTGACAGCACACACACAGG NGG 10 8 17 8 24 0 100 5 10
    240605011655_rphh AGGGTCCTGTGTGTGTGCTGTCACTGGCTCTG(+ACTCAC)GTACCTGCAGGGACATCTTATAAGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605011654_wz88 GGCGTACCCAGGCCAGCACCAGAGGGCGGCAG(+ACTCAC)GTACCCCAGGGAGTGGCTAGGGAGGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605011651_pgge CCTTCGACCAACCTCCCTAGCCACTCCCTGGG(+ACTCAC)GTACCTGCCGCCCTCTGGTGCTGGCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605011650_68rb CCCTGACGGTAGCACCAGGAGATCATGGATAG(+ACTCAC)GATCCCAGGTTCAAGGTCAGGGGCTCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605011648_kvrv GAGTGAGGCTGAGCCCCTGACCTTGAACCTGG(+ACTCAC)GATCCTATCCATGATCTCCTGGTGCTACCGT NGG 10 8 17 8 24 0 100 5 10
    240605011646_n3bv AGTGTACAGCCAGGATAGGCCAAAACCTTTCG(+ACTCAC)GATCCCAGGATGAGACCAAGTCTCCATCAGG NGG 10 8 17 8 24 0 100 5 10
    240605011644_1y7x TAGGACCTGATGGAGACTTGGTCTCATCCTGG(+ACTCAC)GATCCGAAAGGTTTTGGCCTATCCTGGCTGT NGG 10 8 17 8 24 0 100 5 10