Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011556_2rft GTGTGTGTGCGCGCGCGCGCGCATGAATGCAG(+ACTCAC)GTACCCATGGAGGCCCTAAGAAGGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605011554_b7om GGGGATCTGATGCCTTCTTAGGGCCTCCATGG(+ACTCAC)GTACCTGCATTCATGCGCGCGCGCGCGCACA NGG 10 8 17 8 24 0 100 5 10
    240605011551_3xd5 GGGATTCTCGGAGCCAGCCCTTCCTTACCCTG(+ACTCAC)GTACCCTATCCCCACCCCACTGTCTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605011550_sc5x ACTTGGCAGGAAGACAGTGGGGTGGGGATAGG(+ACTCAC)GTACCAGGGTAAGGAAGGGCTGGCTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240605011548_5di8 ACAACTCCCTTGGATGAAGTTATAATCACAGG(+ACTCAC)GATCCATTTTTTTTTAAGTTTAAAAGTCATG NGG 10 8 17 8 24 0 100 5 10
    240605011547_bbj0 AAATTCATGACTTTTAAACTTAAAAAAAAATG(+ACTCAC)GATCCCTGTGATTATAACTTCATCCAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605011546_qjnb CTTGTTGGGGGAGGGACACTCAGACGGGCTTG(+ACTCAC)GTACCTTTAAAGCAGCAATTGTCACTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605011545_rnzs CTAGGCAAGAAGTGACAATTGCTGCTTTAAAG(+ACTCAC)GTACCAAGCCCGTCTGAGTGTCCCTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605011543_w4t3 CTCTTTCCGCCATCCGACCCCTATGGGATGAG(+ACTCAC)GATCCTCGGAGGCCAGGCCTGTGGAGAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605011541_pezr TCCCAGACCTCTCCACAGGCCTGGCCTCCGAG(+ACTCAC)GATCCTCATCCCATAGGGGTCGGATGGCGGA NGG 10 8 17 8 24 0 100 5 10