Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011510_6715 CACAGGCTGCTCCCCACCTCAGTTCTATAAAG(+ACTCAC)GATCCAGACAGGAGAGGCCAAGAGAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605011508_xgnm GCCTCTCTCTCTCTCTTGGCCTCTCCTGTCTG(+ACTCAC)GATCCTTTATAGAACTGAGGTGGGGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605011507_lru5 TCCCAAAGAATGAAGCAATCTATGAAGAGAGG(+ACTCAC)GTACCAGACAAAGATCCAAGACACCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605011505_scuf GGATTTGGGCTGGTGTCTTGGATCTTTGTCTG(+ACTCAC)GTACCCTCTCTTCATAGATTGCTTCATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605011504_t9u9 CATTACTTTTTTCCTGTATATGAAGAAAATTG(+ACTCAC)GTACCCTGTACTTACAATTTATTTATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240605011502_iuz3 ATGCAATAACATAAATAAATTGTAAGTACAGG(+ACTCAC)GTACCAATTTTCTTCATATACAGGAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605011500_9fcy AAGCATAGATGTAATATAAATTTGATTCCCAG(+ACTCAC)GATCCTAGACATCTTAGATAAGTCAATACAA NGG 10 8 17 8 24 0 100 5 10
    240605011459_1our TTTCTTTGTATTGACTTATCTAAGATGTCTAG(+ACTCAC)GATCCTGGGAATCAAATTTATATTACATCTA NGG 10 8 17 8 24 0 100 5 10
    240605011457_g9gs GAAGAAACGGGGGAGACCTTGAATGTTAACAG(+ACTCAC)GATCCATGGTGAGGGTGTTGGGGTTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605011455_bphw ATTTAACACACAACCCCAACACCCTCACCATG(+ACTCAC)GATCCTGTTAACATTCAAGGTCTCCCCCGTT NGG 10 8 17 8 24 0 100 5 10