Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011406_ukco GCATTCCCCGCTGCAGTCACCTCATAGGCTTG(+ACTCAC)GATCCATCAAGACCTACTTCAGGCCCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605011404_wsav TGGAGAGCCGGGGCCTGAAGTAGGTCTTGATG(+ACTCAC)GATCCAAGCCTATGAGGTGACTGCAGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605011402_1doa AGCGCACAGGGAACAGAGGGAGGCCCACCAGG(+ACTCAC)GATCCTGGCTTGCCTACCGAAGGCTATTGCT NGG 10 8 17 8 24 0 100 5 10
    240605011400_soct ACAGCAGCAATAGCCTTCGGTAGGCAAGCCAG(+ACTCAC)GATCCCTGGTGGGCCTCCCTCTGTTCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605011359_a4kq CCGATATTGGATGTGGGCCATTTATGGTGTTG(+ACTCAC)GTACCCACACCAGTTGCCCACTTAACGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605011357_v7ii GCGGGGGGGCGTTAAGTGGGCAACTGGTGTGG(+ACTCAC)GTACCAACACCATAAATGGCCCACATCCAAT NGG 10 8 17 8 24 0 100 5 10
    240605011355_0dgw CCCCATCCACAGCTGTCCAGAAGCTGTCCTGG(+ACTCAC)GATCCTCAGCAATAAAGCACTTTATTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605011354_wu84 TGTATGTAAAAATAAAGTGCTTTATTGCTGAG(+ACTCAC)GATCCCAGGACAGCTTCTGGACAGCTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605011351_6p0k GTGCTAGGGTGTATATAAGCAGTCCTCCCTGG(+ACTCAC)GTACCCGGGATCCCTGTAGCAGCATCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605011348_rh0e GGAACGCCTCGATGCTGCTACAGGGATCCCGG(+ACTCAC)GTACCCAGGGAGGACTGCTTATATACACCCT NGG 10 8 17 8 24 0 100 5 10