Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011329_5eu1 CTCCACTCCTTCTCTACTCTTCTGGGCCCCAG(+ACTCAC)GATCCTCTGCCTAGGGAATGGTGCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240605011328_5czi CCAGCATGGATGGCACCATTCCCTAGGCAGAG(+ACTCAC)GATCCTGGGGCCCAGAAGAGTAGAGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605011326_odjc CAGGCCCAGTGTCACTCTTCTTGCTGCCTTTG(+ACTCAC)GATCCAGATGTGAATTCTCTGCTACCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605011324_ze1q GTGCTGGAGAGGTAGCAGAGAATTCACATCTG(+ACTCAC)GATCCAAAGGCAGCAAGAAGAGTGACACTGG NGG 10 8 17 8 24 0 100 5 10
    240605011323_fpkz ACTTTTGAGGCCACTGCCAGGACCCCAGCCTG(+ACTCAC)GATCCGGGTTTTATCCAGGGACACCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605011320_vtjf CTTCATAGCTGGGTGTCCCTGGATAAAACCCG(+ACTCAC)GATCCAGGCTGGGGTCCTGGCAGTGGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605011319_pmwn CATTCCTGCCAGTTTGGCTAGCTCTAGATAGG(+ACTCAC)GATCCTGCCTTGTATCCTGAGACCCAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605011317_ljm9 TCTGGTCCTCTGGGTCTCAGGATACAAGGCAG(+ACTCAC)GATCCCTATCTAGAGCTAGCCAAACTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605011316_rpl7 GAAGGAATCATACTAGAGAAGAAAAGTCAGAG(+ACTCAC)GTACCACAATCAATAAGAAAACAGTAAATTA NGG 10 8 17 8 24 0 100 5 10
    240605011315_ddep GTTTGTAATTTACTGTTTTCTTATTGATTGTG(+ACTCAC)GTACCTCTGACTTTTCTTCTCTAGTATGATT NGG 10 8 17 8 24 0 100 5 10