Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011227_xd0t TGGCTCAGTAGACCCAAACCAGGACTGTCCTG(+ACTCAC)GATCCACTTTAGTCATGCTACCAGTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605011226_48tw TGTAGCAGCCCACTGGTAGCATGACTAAAGTG(+ACTCAC)GATCCAGGACAGTCCTGGTTTGGGTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240605011224_7wyi TCAAAAAAGCCCCAAGAAATTTTGAAGTGTGG(+ACTCAC)GTACCTCGTTGATTCTTACGCCAGGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605011223_a15k TGATCCTGTGCCCTGGCGTAAGAATCAACGAG(+ACTCAC)GTACCCACACTTCAAAATTTCTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605011222_tvhz CATTTTCCAGTATAATAAAGGTAGCTAAGATG(+ACTCAC)GATCCATATGCAGAAATAGATTACAGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605011221_zyh2 TGGCTAAGGACTGTAATCTATTTCTGCATATG(+ACTCAC)GATCCATCTTAGCTACCTTTATTATACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605011219_jelr TGGAGGAAACACTCTGTTGGAACCCTCCTGTG(+ACTCAC)GATCCACACCTGACTTAGCACTTAAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605011218_qogk GGAGGGTTAGCTTAAGTGCTAAGTCAGGTGTG(+ACTCAC)GATCCACAGGAGGGTTCCAACAGAGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605011215_xcfp TTGGGACCTGGCATCAGGACCTCTCCACCCAG(+ACTCAC)GATCCTCCATGCCACATGGTCACTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605011214_54mg CCTGAGGAGAGCAGTGACCATGTGGCATGGAG(+ACTCAC)GATCCTGGGTGGAGAGGTCCTGATGCCAGGT NGG 10 8 17 8 24 0 100 5 10