Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011256_co6w ATACAGTGCCTTCACCATGGTCACAAATTCTG(+ACTCAC)GTACCTTTGTGCAAAAGTTGTGGTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605011255_h3ij AGCAAAAAACCAACCACAACTTTTGCACAAAG(+ACTCAC)GTACCAGAATTTGTGACCATGGTGAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605011254_zw2m CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+ACTCAC)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605011252_0iwm GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+ACTCAC)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605011251_ylbw TGGGAGCTGGCATCAACAGTATGGTCCTATTG(+ACTCAC)GATCCTAGCAGACCTTTTTGCTACAGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605011249_8u70 GTATCCTCCACTGTAGCAAAAAGGTCTGCTAG(+ACTCAC)GATCCAATAGGACCATACTGTTGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605011248_lfh6 AGAGGCTTTCTGTGCTACACTGTCTTAGCTCG(+ACTCAC)GATCCGGTGGGGGTTTGGCTGTCCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605011246_1osq GGGCAGGCCACAGGACAGCCAAACCCCCACCG(+ACTCAC)GATCCGAGCTAAGACAGTGTAGCACAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605011245_be5k CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+ACTCAC)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605011243_ye36 GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+ACTCAC)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10