Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011346_dkv3 GTGCTGGGAGCCCGCCAGGGCTGCTAAACCAG(+ACTCAC)GTACCTCTCTCTGTCTGGAGGTTTGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605011345_k5kp CAACTTCTTTCCAAACCTCCAGACAGAGAGAG(+ACTCAC)GTACCTGGTTTAGCAGCCCTGGCGGGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605011344_8onz AGTTCAGATGGGAAATTTAGGAGAGTTTTTGG(+ACTCAC)GTACCCGAGTTACTTCACATTATGTAGTACC NGG 10 8 17 8 24 0 100 5 10
    240605011342_na1t TTTGAGGTACTACATAATGTGAAGTAACTCGG(+ACTCAC)GTACCCAAAAACTCTCCTAAATTTCCCATCT NGG 10 8 17 8 24 0 100 5 10
    240605011340_ytuf TTTTTCCACAGGATAAAGAACATCCAATTTGG(+ACTCAC)GTACCAGAAAGGCTGACATGAAAATTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240605011338_goru TAAGATAAGCAATTTTCATGTCAGCCTTTCTG(+ACTCAC)GTACCCAAATTGGATGTTCTTTATCCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605011336_wv3n GTGTATATAAGCAGTCCTCCCTGGGTACCCGG(+ACTCAC)GATCCCTGTAGCAGCATCGAGGCGTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605011334_2hvj TTATTGCAGGAACGCCTCGATGCTGCTACAGG(+ACTCAC)GATCCCGGGTACCCAGGGAGGACTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240605011333_91qx CCATCCATGCTGGGTGCCATCCCACCTCAATG(+ACTCAC)GTACCAATATAACCCCCAACAGACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605011332_rei9 GCCTTTATAAATGTCTGTTGGGGGTTATATTG(+ACTCAC)GTACCATTGAGGTGGGATGGCACCCAGCATG NGG 10 8 17 8 24 0 100 5 10