Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011454_sjse CCTCCGTGTCGTGCAGACTCAGAATAGACTTG(+ACTCAC)GATCCTTTCTCTCTGATTCTCTGGTGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605011453_0zyr GGAGGGTGGGCACCAGAGAATCAGAGAGAAAG(+ACTCAC)GATCCAAGTCTATTCTGAGTCTGCACGACAC NGG 10 8 17 8 24 0 100 5 10
    240605011451_bbb4 GAAGTAGGCTAAGTGGAGGAATGCTCTCCAAG(+ACTCAC)GATCCACCTTGTCCCCGCCCCTGCAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605011450_74db AGTGGCTGAGCTGCAGGGGCGGGGACAAGGTG(+ACTCAC)GATCCTTGGAGAGCATTCCTCCACTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605011447_ndrs TTATAGCCTTTCGCCTCTCCTAGCTTCTCCCG(+ACTCAC)GATCCTCACCCCTACCCACACAACTTCATAT NGG 10 8 17 8 24 0 100 5 10
    240605011446_oc2e AAAGGATATGAAGTTGTGTGGGTAGGGGTGAG(+ACTCAC)GATCCGGGAGAAGCTAGGAGAGGCGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605011444_by62 TTCCGTATGTTAGCTCAGTCTTGTATTCCTGG(+ACTCAC)GATCCATCCCATTTGGTCATGGTGTCTAATC NGG 10 8 17 8 24 0 100 5 10
    240605011443_ubai AAAATGATTAGACACCATGACCAAATGGGATG(+ACTCAC)GATCCCAGGAATACAAGACTGAGCTAACATA NGG 10 8 17 8 24 0 100 5 10
    240605011441_fw36 ACCCGGCCTGGCTCTTGTGTTTTGACTAATTG(+ACTCAC)GATCCCAGGTGCAAGCCTTGCTTGGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605011439_idin AGGGGTTGTGACCAAGCAAGGCTTGCACCTGG(+ACTCAC)GATCCAATTAGTCAAAACACAAGAGCCAGGC NGG 10 8 17 8 24 0 100 5 10