Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011420_jvc4 CTCCTACTTTGTTCCCAGTAGTCCCGCGTAGG(+ACTCAC)GATCCTGACCCGGCAGTGTGCTAAGCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605011419_8j7x CTGGCCTCTTGCTTAGCACACTGCCGGGTCAG(+ACTCAC)GATCCCTACGCGGGACTACTGGGAACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605011418_w3n8 CCGAGGGGGAAATTATGTCCACGGCCTCGCTG(+ACTCAC)GTACCATGGAAGACTCATGCCCCCTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605011416_46cb GGGAAATGGGCAGGGGGCATGAGTCTTCCATG(+ACTCAC)GTACCAGCGAGGCCGTGGACATAATTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605011415_0v91 GCACATGGATCTCACCCGTATTCTGTTGTATG(+ACTCAC)GTACCTCGGTACATCTTTGTGGTTGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605011413_i0fg CAGTTTTAACACAACCACAAAGATGTACCGAG(+ACTCAC)GTACCATACAACAGAATACGGGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240605011411_wia0 GAGTAAATAGGGGACATGGTGCATTTTTGCTG(+ACTCAC)GATCCTGTGAAAGGTTTATTTAGAGCCGATT NGG 10 8 17 8 24 0 100 5 10
    240605011410_p0xx TCTGGAATCGGCTCTAAATAAACCTTTCACAG(+ACTCAC)GATCCAGCAAAAATGCACCATGTCCCCTATT NGG 10 8 17 8 24 0 100 5 10
    240605011409_1p3r TAGTTTTGAGGGCTTACACAAGCTTATTCTTG(+ACTCAC)GTACCACAGGAATTTGGTTAAATGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605011407_qrr0 CAAAGGAAATTCCATTTAACCAAATTCCTGTG(+ACTCAC)GTACCAAGAATAAGCTTGTGTAAGCCCTCAA NGG 10 8 17 8 24 0 100 5 10