Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011540_ncij GTATGAGTGGCTCTGAAAAGCCATAAGGATAG(+ACTCAC)GTACCTACATGAGAAGGACACCTTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605011539_l3lk CTAGCGAAAGGAAAGGTGTCCTTCTCATGTAG(+ACTCAC)GTACCTATCCTTATGGCTTTTCAGAGCCACT NGG 10 8 17 8 24 0 100 5 10
    240605011537_yb7l GGAGTTTTTATACGTGCATATGCATAAGCAGG(+ACTCAC)GATCCTTGGGTTACATGACAGGGGAGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605011534_8jm7 CTCTTGTCACCTCCCCTGTCATGTAACCCAAG(+ACTCAC)GATCCCTGCTTATGCATATGCACGTATAAAA NGG 10 8 17 8 24 0 100 5 10
    240605011533_a85t ACAGATACAAATCTGAATTGTGTAAGACTCAG(+ACTCAC)GATCCTGCTTCCAGCCCTGTGTGTAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605011532_1emt TGCTGACAACCTACACACAGGGCTGGAAGCAG(+ACTCAC)GATCCTGAGTCTTACACAATTCAGATTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605011531_k0qe TGGTCCACAAGCTGCAAGCTCCCTCCGCATAG(+ACTCAC)GTACCTGTCTGAGGAGCCTTGTCCCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605011529_4asy AGGCTGCCTCAGGGACAAGGCTCCTCAGACAG(+ACTCAC)GTACCTATGCGGAGGGAGCTTGCAGCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605011528_0glv AGTGGCTTTCTCTGTGGGCCAGAACTCTTAGG(+ACTCAC)GTACCAGAGCCTCCTGAGTGTGAGCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605011526_1euf CTTTCTCTTCTGCTCACACTCAGGAGGCTCTG(+ACTCAC)GTACCCTAAGAGTTCTGGCCCACAGAGAAAG NGG 10 8 17 8 24 0 100 5 10