Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011610_vzzz TCCTGTTCAAATGTACCTCAGTGGAGAGAGAG(+ACTCAC)GATCCTCGCTTCTACACTGTGTGGCCTGACG NGG 10 8 17 8 24 0 100 5 10
    240605011609_t6jr ACTTCCGTCAGGCCACACAGTGTAGAAGCGAG(+ACTCAC)GATCCTCTCTCTCCACTGAGGTACATTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605011607_8v2c CCTGTAGAAGGCAAATGAGACACTGATTAGTG(+ACTCAC)GATCCCTGCAGAACTGGGTCCTAGATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605011606_pxhg AAGCCTTTGGATCTAGGACCCAGTTCTGCAGG(+ACTCAC)GATCCACTAATCAGTGTCTCATTTGCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605011604_d967 GCTGTATCAGAGGCAGCTTTCATCTTAGCATG(+ACTCAC)GTACCCAGAAAAGGTTGGACATCAGCAAACA NGG 10 8 17 8 24 0 100 5 10
    240605011603_8i7r TTTCCTGTTTGCTGATGTCCAACCTTTTCTGG(+ACTCAC)GTACCATGCTAAGATGAAAGCTGCCTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605011602_1t76 GAGAAAGAGCGGAGTCTGTGGTAGGAAGAACG(+ACTCAC)GTACCCTGTTTGCCTTCAAGTGTGAGTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605011600_v7l7 CGCTCAAACACTCACACTTGAAGGCAAACAGG(+ACTCAC)GTACCGTTCTTCCTACCACAGACTCCGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605011559_ir32 CTCGCACACTGCAGGGTCCTTGCCCAGATGAG(+ACTCAC)GTACCAAAGAGGAGCTGGCCCAGATGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605011558_a3yo AAACGATTCACATCTGGGCCAGCTCCTCTTTG(+ACTCAC)GTACCTCATCTGGGCAAGGACCCTGCAGTGT NGG 10 8 17 8 24 0 100 5 10