Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011907_fh5l TGAGTGCTTGAGGAGGCCAGAAGAGGGTGTTG(+ACTCAC)GATCCCCTGGAGCTGAAGTAATGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240605011904_1ze7 GCTTACAATGGCCCATTACTTCAGCTCCAGGG(+ACTCAC)GATCCAACACCCTCTTCTGGCCTCCTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605011902_flz8 AGTCCCAATTTCAGGGTGGCACAAATTGTATG(+ACTCAC)GATCCAGCTCTTGGATCACACAATCGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605011901_0cnm GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+ACTCAC)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 10 8 17 8 24 0 100 5 10
    240605011900_yj3n TCAACCCTGAGGTGTCTGTCCACTATCTGAAG(+ACTCAC)GATCCAATTATTTCTAGCTCCCATCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605011859_54bg AGGGTAGGAGGGATGGGAGCTAGAAATAATTG(+ACTCAC)GATCCTTCAGATAGTGGACAGACACCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605011857_525j TCATCTGGGAAAGCACTGATTACACTTTACAG(+ACTCAC)GTACCTGTCAGCCCTGAGCTTCACAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240605011856_netq TCGTGTTTGTCTGTGAAGCTCAGGGCTGACAG(+ACTCAC)GTACCTGTAAAGTGTAATCAGTGCTTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605011855_4cl1 ACTCAATGCAAACCTAACTGGCTATTAGCTCG(+ACTCAC)GATCCTTTGAATCTTTGAGTTACAAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605011854_03rb TGAAAGAGCTTTTGTAACTCAAAGATTCAAAG(+ACTCAC)GATCCGAGCTAATAGCCAGTTAGGTTTGCAT NGG 10 8 17 8 24 0 100 5 10