Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605011938_9xph TTTGGTGCTCAGTAAAGGCCTGCAGAGCCTCG(+ACTCAC)GATCCCGTTTGTGCTTTGAAGTCCTCACATG NGG 10 8 17 8 24 0 100 5 10
    240605011936_qewy AATTGCATGTGAGGACTTCAAAGCACAAACGG(+ACTCAC)GATCCGAGGCTCTGCAGGCCTTTACTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605011935_6hom ATGTTCCTACCGCGGATGCTTTCAGTGCATAG(+ACTCAC)GATCCTCAAGCAACATGAACCTGAAATAAGC NGG 10 8 17 8 24 0 100 5 10
    240605011934_d4yu ACTGGGCTTATTTCAGGTTCATGTTGCTTGAG(+ACTCAC)GATCCTATGCACTGAAAGCATCCGCGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605011932_km0a TCAATAATTTTTATCAGATTCTTCTCTGTAGG(+ACTCAC)GTACCTTTCTCATATGTTCTGATTATTTACA NGG 10 8 17 8 24 0 100 5 10
    240605011931_bn63 TACAGTGTAAATAATCAGAACATATGAGAAAG(+ACTCAC)GTACCCTACAGAGAAGAATCTGATAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240605011929_1wha GATCTGTATGTATACCACATAATGCGTGCCAG(+ACTCAC)GTACCCACAGAGGTCATAAGAGGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605011927_7g4a AGGGGATCTTACCCCTCTTATGACCTCTGTGG(+ACTCAC)GTACCTGGCACGCATTATGTGGTATACATAC NGG 10 8 17 8 24 0 100 5 10
    240605011926_itov AGGTACACTGAGCCCCATGCTGGAGTGAGTGG(+ACTCAC)GTACCTGGTCATGAGAGATGCCCAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605011924_ntw5 TGCAGGCTGAGCTGGGCATCTCTCATGACCAG(+ACTCAC)GTACCCACTCACTCCAGCATGGGGCTCAGTG NGG 10 8 17 8 24 0 100 5 10