Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012011_5wwu CACAGTATGCACACAGACAACTGTGAATTCAG(+ACTCAC)GTACCCTTCCACAAGCCTTCCCTCTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240605012009_y98q TGGAAAGTACAAGAGGGAAGGCTTGTGGAAGG(+ACTCAC)GTACCTGAATTCACAGTTGTCTGTGTGCATA NGG 10 8 17 8 24 0 100 5 10
    240605012008_i6qi TGCTATCCTGGGTAAGAACGCCCTGAGTACGG(+ACTCAC)GTACCATGCTCTGTTTACTTTAGGATCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605012006_x3il GGTGCTGGAGATCCTAAAGTAAACAGAGCATG(+ACTCAC)GTACCCGTACTCAGGGCGTTCTTACCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605012004_xsvz TAAAAACCCTCATCAAACTACTTCATTTCCAG(+ACTCAC)GATCCCCACATTTCCACTTTTGTGAAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605012002_1xvq TTTTAACAGCTTCACAAAAGTGGAAATGTGGG(+ACTCAC)GATCCTGGAAATGAAGTAGTTTGATGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605012000_6gad AGATGACTCTCCATGAGAAGACTTGAAAACTG(+ACTCAC)GTACCAGGAGTGGGCTGAAGATTCCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240605011959_gapq ATGACGTCATGGGAATCTTCAGCCCACTCCTG(+ACTCAC)GTACCAGTTTTCAAGTCTTCTCATGGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605011957_m3ex AGTCCCTCAAACTATACTGATGATCAAATAAG(+ACTCAC)GATCCGAACCACTACTAGAAATGCCCAACTA NGG 10 8 17 8 24 0 100 5 10
    240605011956_1dut AGATCTAGTTGGGCATTTCTAGTAGTGGTTCG(+ACTCAC)GATCCTTATTTGATCATCAGTATAGTTTGAG NGG 10 8 17 8 24 0 100 5 10