Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012153_i31s GAGCCGCGTGACTGTACGTGTTGAAGGGGGCG(+ACTCAC)GTACCTGTTCACAGCACAAGTTTATGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605012152_qb3u CAGAGCTCAACATAAACTTGTGCTGTGAACAG(+ACTCAC)GTACCGCCCCCTTCAACACGTACAGTCACGC NGG 10 8 17 8 24 0 100 5 10
    240605012151_d7wv ACTCCCACACTCCACGTACTTGTTCGGAGCAG(+ACTCAC)GTACCACTGACTACAAAGTCATCAGTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605012150_z2df TATCACAATAACTGATGACTTTGTAGTCAGTG(+ACTCAC)GTACCTGCTCCGAACAAGTACGTGGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605012149_e8j3 CAGACCCTTTTACATCACAAATGGGAAGACTG(+ACTCAC)GTACCCCAAAGGCTGGCTGGAATGCTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605012147_ayep AGTGGCCTTAAGCATTCCAGCCAGCCTTTGGG(+ACTCAC)GTACCAGTCTTCCCATTTGTGATGTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605012146_p7vb TGCTGATGACTAACTTTTGCAGATAAGCATAG(+ACTCAC)GTACCTGTGTTCAACAGATGCCAGCAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605012144_qocs AAGATACTTCTGCTGGCATCTGTTGAACACAG(+ACTCAC)GTACCTATGCTTATCTGCAAAAGTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605012143_1qq7 ACACACACCCCAGGTCTGTACCAGGACTTTGG(+ACTCAC)GATCCCAAGCTGTTTTCAAGCTGTTTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605012141_5nwz TGAGCCTCAGAAACAGCTTGAAAACAGCTTGG(+ACTCAC)GATCCCAAAGTCCTGGTACAGACCTGGGGTG NGG 10 8 17 8 24 0 100 5 10