Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012734_xlh3 ATGACTGATATCTATAGCCCAGGAACCGCCTG(+ACTCAC)GATCCAGGATTGTGAAACTAAGCCCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605012732_1k87 GTCCCTCCCACGGGCTTAGTTTCACAATCCTG(+ACTCAC)GATCCAGGCGGTTCCTGGGCTATAGATATCA NGG 10 8 17 8 24 0 100 5 10
    240605012731_2hw8 TCCTCTACAAGGATACTGTGGCTAATGCACAG(+ACTCAC)GTACCCTGATTGCAGAGAAAACTCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605012729_rbg0 TATCTATGCCCAGAGTTTTCTCTGCAATCAGG(+ACTCAC)GTACCTGTGCATTAGCCACAGTATCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605012728_gbmg AAGGTACTACCGGCTATAGCCACAGGGATCCG(+ACTCAC)GTACCATTGAGATCCCTTCATAAAATATGGA NGG 10 8 17 8 24 0 100 5 10
    240605012726_laue CTTGCTCCATATTTTATGAAGGGATCTCAATG(+ACTCAC)GTACCGGATCCCTGTGGCTATAGCCGGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605012724_0v3z TGCTCCCGCCTATCTTGCTCCTTAACACTGGG(+ACTCAC)GATCCGATAGAGAAATGACACAAGGTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605012723_hu88 ACACACTGAAACCTTGTGTCATTTCTCTATCG(+ACTCAC)GATCCCCAGTGTTAAGGAGCAAGATAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605012721_upn7 GCTCCTAAGGTACTACCGGCTATAGCCACAGG(+ACTCAC)GATCCGGTACCATTGAGATCCCTTCATAAAA NGG 10 8 17 8 24 0 100 5 10
    240605012719_ylz7 CCATATTTTATGAAGGGATCTCAATGGTACCG(+ACTCAC)GATCCCTGTGGCTATAGCCGGTAGTACCTTA NGG 10 8 17 8 24 0 100 5 10