Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012645_fnna GCCCATGCCACTGTGGCCCTTAATATGCACAG(+ACTCAC)GTACCCAAGGGTGGCACAAAAGCATGTACCT NGG 10 8 17 8 24 0 100 5 10
    240605012643_78fa ACTTGAGGTACATGCTTTTGTGCCACCCTTGG(+ACTCAC)GTACCTGTGCATATTAAGGGCCACAGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605012642_pszd GAAAAAGAAATGAAGATTTCCCTCCTCAAATG(+ACTCAC)GTACCCTGGGGACAGCTTGTCTAGTTATGTG NGG 10 8 17 8 24 0 100 5 10
    240605012639_vwd3 AATGTCACATAACTAGACAAGCTGTCCCCAGG(+ACTCAC)GTACCATTTGAGGAGGGAAATCTTCATTTCT NGG 10 8 17 8 24 0 100 5 10
    240605012638_y3yc AATTCACACCCTTGAAGAAAAGGTTATTACAG(+ACTCAC)GATCCAAAAGCAGTCCAGAAGGAACTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605012637_t3cy AGAACCTTGCAGTTCCTTCTGGACTGCTTTTG(+ACTCAC)GATCCTGTAATAACCTTTTCTTCAAGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605012636_mckl CAAGAGTTTAAGCCCTGCCCCATCTCTACTTG(+ACTCAC)GTACCCAATGACACTAACAAGGAAGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605012634_iuaz AGATAAAACAGCTTCCTTGTTAGTGTCATTGG(+ACTCAC)GTACCAAGTAGAGATGGGGCAGGGCTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240605012633_nsoz CATCCACAGTTATGTATAAACCCACTGCAGTG(+ACTCAC)GATCCCAGGACACAAACCCAGAAGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605012630_j6xq CCTGAGAAATTCCTTCTGGGTTTGTGTCCTGG(+ACTCAC)GATCCACTGCAGTGGGTTTATACATAACTGT NGG 10 8 17 8 24 0 100 5 10