Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605012718_jvmq GGAGCCACAAGGAGTGGGCTTCATGCATTATG(+ACTCAC)GATCCCGACCCCCGCCACTGTGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605012716_44zx TCCTGGAGCAAGCCCACAGTGGCGGGGGTCGG(+ACTCAC)GATCCATAATGCATGAAGCCCACTCCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605012713_iimz CCAGCAAGAAGGGGGAGCAGGGGACGAGGGGG(+ACTCAC)GTACCTATCCTTCCTGAACGGACAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605012712_r88z CATCACCCCCATTGTCCGTTCAGGAAGGATAG(+ACTCAC)GTACCCCCCTCGTCCCCTGCTCCCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605012711_js0k AAAGTATTTCATTATGCTGGCATGCCCAGTAG(+ACTCAC)GATCCCCTGAGGCCCAGATGGGCCACATGTA NGG 10 8 17 8 24 0 100 5 10
    240605012709_xzw6 TGGTATACATGTGGCCCATCTGGGCCTCAGGG(+ACTCAC)GATCCTACTGGGCATGCCAGCATAATGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605012708_5ypi CCACCTCACACCCAATATGTCCAAAGTCTCAG(+ACTCAC)GATCCAGGAATAAACAAAGCCGGGAGAATCT NGG 10 8 17 8 24 0 100 5 10
    240605012706_zrft CACGCAGATTCTCCCGGCTTTGTTTATTCCTG(+ACTCAC)GATCCTGAGACTTTGGACATATTGGGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605012703_2df2 GGCGATGGCGCAGATGGTGCGTTCAAACCCGG(+ACTCAC)GATCCCAGCTGTCCACTACACCAATGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605012701_ak98 CGCCATGTCCCATTGGTGTAGTGGACAGCTGG(+ACTCAC)GATCCCGGGTTTGAACGCACCATCTGCGCCA NGG 10 8 17 8 24 0 100 5 10