Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010836_prg1 TATAATTTATGCAGAGAAATTTCACTTACATG(+ACTCAC)GTACCTTTTCCAGTCTCAACATCTCATCATA NGG 10 8 17 8 24 0 100 5 10
    240605010834_256k CATTTTATGATGAGATGTTGAGACTGGAAAAG(+ACTCAC)GTACCATGTAAGTGAAATTTCTCTGCATAAA NGG 10 8 17 8 24 0 100 5 10
    240605010833_0egu GGAAGGTTGGTCCCACAAGATGCACTGAAATG(+ACTCAC)GATCCCCACCCAATAAAGATGGTATTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605010831_w1hp CATATGTAACAATACCATCTTTATTGGGTGGG(+ACTCAC)GATCCATTTCAGTGCATCTTGTGGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605010830_nspm TACATAAACCAATGCTTGAAGATGCTGCAAAG(+ACTCAC)GATCCTCTAGTGATTCCTGATGAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240605010829_o6zw CTATTCTGATTCCTCATCAGGAATCACTAGAG(+ACTCAC)GATCCTTTGCAGCATCTTCAAGCATTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605010828_o40r TCCTGTATCTTGCCTTTGTACAAGAGCATATG(+ACTCAC)GTACCTTTTTGAATAAAATAACATTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240605010826_1kl6 TAAATATGTTAAATGTTATTTTATTCAAAAAG(+ACTCAC)GTACCATATGCTCTTGTACAAAGGCAAGATA NGG 10 8 17 8 24 0 100 5 10
    240605010824_wnlf AGGAAGCTCTGGCAATTCTGGTTCTTAACCAG(+ACTCAC)GATCCAGGAGGCATCACTGGGAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605010823_sb23 TTGAAATCACACTTTCCCAGTGATGCCTCCTG(+ACTCAC)GATCCTGGTTAAGAACCAGAATTGCCAGAGC NGG 10 8 17 8 24 0 100 5 10