Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605010821_qfc0 GATCGTTGAATCTCTCTCATCCCAGTGCAATG(+ACTCAC)GATCCACAAATCCGTGGGATGCAAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605010820_9ub5 AGTTCTCTGCTTTTGCATCCCACGGATTTGTG(+ACTCAC)GATCCATTGCACTGGGATGAGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240605010818_k4rs ACAAATAGCTCCCCAGGATCTGATTAAGATAG(+ACTCAC)GATCCGGGCCATACCATTTATTATCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605010816_n88p AAAATGTCCTTGATAATAAATGGTATGGCCCG(+ACTCAC)GATCCTATCTTAATCAGATCCTGGGGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605010815_jo5v ATTTGAAATAAAGGTTTTAATTCTGAAGTAGG(+ACTCAC)GATCCTTAAATAAGGTCTAATCCAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605010813_oel6 GGAGATATAAATTGGATTAGACCTTATTTAAG(+ACTCAC)GATCCCTACTTCAGAATTAAAACCTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605010812_tqge AGGGAACCCAGGGTCACCTGCAGGTGACAGAG(+ACTCAC)GTACCCGCCTCCACTTAATGTCCATCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605010810_50yr ACCCCAGCTTGATGGACATTAAGTGGAGGCGG(+ACTCAC)GTACCTCTGTCACCTGCAGGTGACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605010809_ols9 GGTGGGCTAAGTTGCCAAAAACGACAATCAGG(+ACTCAC)GATCCAATAAGCAATCTTGGTCACTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605010807_0mmg AAATTTACTTAAGTGACCAAGATTGCTTATTG(+ACTCAC)GATCCCTGATTGTCGTTTTTGGCAACTTAGC NGG 10 8 17 8 24 0 100 5 10