Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005928_amb2 CATCCCCACTTATAGCCAATGTACCTTGGAAG(+ACTCAC)GTACCAGGCAGGCTGCTTCAGGGATGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605005927_og2x ATTTACACAGCATCCCTGAAGCAGCCTGCCTG(+ACTCAC)GTACCTTCCAAGGTACATTGGCTATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605005925_05uc CCCGACCTATTTTGGTTTTGGGGGGCATACGG(+ACTCAC)GTACCTTTTGTGTTATTAGTTTATGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605005924_irr0 ATCTTAATATACATAAACTAATAACACAAAAG(+ACTCAC)GTACCCGTATGCCCCCCAAAACCAAAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605005922_s6v1 TTTCAGTGGTATGTCAAGTACAAGGCCTCTGG(+ACTCAC)GATCCACACAAGCAGATGTTCTTGGAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605005921_1sst ATGGATGATTTCCAAGAACATCTGCTTGTGTG(+ACTCAC)GATCCCAGAGGCCTTGTACTTGACATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605005919_43po AGGCCCAGGATCTAGATTGCAGAGAACTTGAG(+ACTCAC)GATCCATGACTTAGCCAGCAGGTGTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605005918_21i2 TGGAGCAAAACACACCTGCTGGCTAAGTCATG(+ACTCAC)GATCCTCAAGTTCTCTGCAATCTAGATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605005917_pxx0 GGCCCTGCTCAGATGGATGCAGAGCATTGAGG(+ACTCAC)GATCCAGATGAGGTGTGGTCCAGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605005915_k9d4 GATGCCCTCTTCCCTGGACCACACCTCATCTG(+ACTCAC)GATCCCTCAATGCTCTGCATCCATCTGAGCA NGG 10 8 17 8 24 0 100 5 10