Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005843_y1jt CCCACACACACAATATTTTGCTACCTTTGTAG(+ACTCAC)GTACCCTTCCTAAGAAGCTGAAATGTATGAC NGG 10 8 17 8 24 0 100 5 10
    240605005841_rkjx ATGGGGTCATACATTTCAGCTTCTTAGGAAGG(+ACTCAC)GTACCTACAAAGGTAGCAAAATATTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605005840_m2pl GTACCCATGTACAATTATATTCACATTCTGAG(+ACTCAC)GTACCGGAGATTGGGGTCTCAGAGTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605005838_czix CCCAGATTTGTACTCTGAGACCCCAATCTCCG(+ACTCAC)GTACCTCAGAATGTGAATATAATTGTACATG NGG 10 8 17 8 24 0 100 5 10
    240605005836_bh0a TGCTCTGAGGACCCGTATGACGGGTACTCTGG(+ACTCAC)GTACCAGGCTACCAGGGACACACCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605005834_sg76 AGAGCATCACTTGGTGTGTCCCTGGTAGCCTG(+ACTCAC)GTACCCAGAGTACCCGTCATACGGGTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605005833_l323 GGTCACATCATCTTAACGTAAACATCAATAAG(+ACTCAC)GTACCAACATTGTTAATGGAGGAAAAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605005832_w3ss TTTGGGTTGCTTTTCCTCCATTAACAATGTTG(+ACTCAC)GTACCTTATTGATGTTTACGTTAAGATGATG NGG 10 8 17 8 24 0 100 5 10
    240605005830_9ufz TGTCACGCAGAATGTGGTGTCCTGACTCAGTG(+ACTCAC)GATCCCCGTGCCTGCCGGGATCTGTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605005828_9zic AAGTGGTGTTCACAGATCCCGGCAGGCACGGG(+ACTCAC)GATCCACTGAGTCAGGACACCACATTCTGCG NGG 10 8 17 8 24 0 100 5 10