Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005755_nr0g TGTTTGCCTTGTTCTCCAGGGGGGCTGCTGGG(+ACTCAC)GTACCCTGGCTTGGGGTGCCCTGTCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605005753_2ak7 GCTTCAACCAAGACAGGGCACCCCAAGCCAGG(+ACTCAC)GTACCCCAGCAGCCCCCCTGGAGAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605005751_a440 AGTGCAGTCCAGGCTAGCCTTGATCTTAACGG(+ACTCAC)GATCCCTGTGCTGACTCAGCCTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605005749_0vmm AAAAAAAAAAAAAAAGGCTGAGTCAGCACAGG(+ACTCAC)GATCCCGTTAAGATCAAGGCTAGCCTGGACT NGG 10 8 17 8 24 0 100 5 10
    240605005747_dw7q GCCAGGGACTTTTCTCCTTCTCACTTGGCCAG(+ACTCAC)GTACCCCTAACTCCTGCTTGCAGAGGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605005745_g5e5 TCATTGGATTCCTCTGCAAGCAGGAGTTAGGG(+ACTCAC)GTACCTGGCCAAGTGAGAAGGAGAAAAGTCC NGG 10 8 17 8 24 0 100 5 10
    240605005744_wvns TATGAGACCTGACAGTTGAATGGGTGGCGTAG(+ACTCAC)GATCCGTGAAAATTTTGGGCAGTCTTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605005743_ggnw CGGGCTCCACAAGACTGCCCAAAATTTTCACG(+ACTCAC)GATCCTACGCCACCCATTCAACTGTCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605005741_n986 CAAGAGGCCAGCCACTGAAGCCAGTGGCCTGG(+ACTCAC)GTACCCAGGGGCCACTCCTTCCTCATAGACT NGG 10 8 17 8 24 0 100 5 10
    240605005737_z5sp GGCCCAGTCTATGAGGAAGGAGTGGCCCCTGG(+ACTCAC)GTACCCAGGCCACTGGCTTCAGTGGCTGGCC NGG 10 8 17 8 24 0 100 5 10