Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005628_f1fb CACACACCTGCTTCCTGGGTCCTATGGACAGG(+ACTCAC)GTACCCACCATACCCTTCCTATCCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605005627_3qjt CCTGGGAGGGAGGGATAGGAAGGGTATGGTGG(+ACTCAC)GTACCCTGTCCATAGGACCCAGGAAGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605005625_d635 GGTTGGCTTCGCTGTTTTCCGGTTCCGTTCCG(+ACTCAC)GATCCAGCCTAGGCCTCACACACACCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605005623_ymv2 GCCCCTGCCCGGTGTGTGTGAGGCCTAGGCTG(+ACTCAC)GATCCGGAACGGAACCGGAAAACAGCGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605005621_5vfy CCAGCCTCTTGAAAGTTAGGATGGGGTGGGAG(+ACTCAC)GATCCCACTTTTCTTATTGGCTGGAGGACAA NGG 10 8 17 8 24 0 100 5 10
    240605005619_lvhy GCACATTGTCCTCCAGCCAATAAGAAAAGTGG(+ACTCAC)GATCCTCCCACCCCATCCTAACTTTCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605005617_li4m TCTTACTCCTGAGTATCCCAGGAGCTCAAAGG(+ACTCAC)GTACCGGGTGGTGTGCAGCGGGGTTAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605005615_xgcw TGGCCCCTTCTAACCCCGCTGCACACCACCCG(+ACTCAC)GTACCCTTTGAGCTCCTGGGATACTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605005613_0lnc GCTTCAGTGGAGGCCGGCCGGGGGAGGGAAGG(+ACTCAC)GATCCTATATTTCCTGCTGACTCTTATTAGG NGG 10 8 17 8 24 0 100 5 10
    240605005612_wmrj CTGACCCTAATAAGAGTCAGCAGGAAATATAG(+ACTCAC)GATCCCTTCCCTCCCCCGGCCGGCCTCCACT NGG 10 8 17 8 24 0 100 5 10