Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005516_hhvr GGAGGGCTCATCGACAAGTAGCCCCCAGCTGG(+ACTCAC)GATCCGCAACATCCACCTCCATCTCCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605005515_0qiw ACGGATCTGAGGAGATGGAGGTGGATGTTGCG(+ACTCAC)GATCCCAGCTGGGGGCTACTTGTCGATGAGC NGG 10 8 17 8 24 0 100 5 10
    240605005513_h7wj TCCTCCTTCCCCTGGCTAGCACTCTGGTCAGG(+ACTCAC)GTACCCCTTTATTCTGCTGCAGAGATCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605005511_g9rz TGTGTTTAAGATCTCTGCAGCAGAATAAAGGG(+ACTCAC)GTACCCTGACCAGAGTGCTAGCCAGGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605005510_tjeh CCATTCCCCCATTCGACATGGGATTTCACCAG(+ACTCAC)GATCCACCTATATCCCCCTTTTTTTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605005508_7ylw AAATATCTTTCAAAAAAAGGGGGATATAGGTG(+ACTCAC)GATCCTGGTGAAATCCCATGTCGAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605005506_w58w GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+ACTCAC)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240605005505_ck69 CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+ACTCAC)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240605005503_pq2x GTGTATGTTAGTGGCAGGAAATTCAGAGTTGG(+ACTCAC)GATCCCTGATAGGAGGCAGATCCTGGACTGG NGG 10 8 17 8 24 0 100 5 10
    240605005501_esns CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+ACTCAC)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10