Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005415_rdgr ATTAGAAAGGGTGGCAGGTCAAGTCGAGGAAG(+ACTCAC)GTACCATCAGCCCAAGCAAAAAGCCCGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605005414_ixxe AGCCAAACCCGGGCTTTTTGCTTGGGCTGATG(+ACTCAC)GTACCTTCCTCGACTTGACCTGCCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605005412_23wm AATACATTTTATGCCGGGGGTAGGGGGACAGG(+ACTCAC)GATCCGTGCTATTGTGGAGCAGCACTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240605005411_uavq TTCAGGTTACAGTGCTGCTCCACAATAGCACG(+ACTCAC)GATCCCTGTCCCCCTACCCCCGGCATAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605005409_lnkd AACCCCTCAAAGCAGAGAGGTCTGGGGAACAG(+ACTCAC)GTACCCCCTAGCTCCGGATACCACTGCGTCC NGG 10 8 17 8 24 0 100 5 10
    240605005407_thqd GGGTGGGACGCAGTGGTATCCGGAGCTAGGGG(+ACTCAC)GTACCTGTTCCCCAGACCTCTCTGCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605005404_18t9 AGCTTTGCCCTTACTGATCTTACTTTCCCGAG(+ACTCAC)GTACCTACGATGCTCTCTGATGCCAGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605005403_sx0a CCAAGATCTGCTGGCATCAGAGAGCATCGTAG(+ACTCAC)GTACCTCGGGAAAGTAAGATCAGTAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605005401_jdkm TGGTCACCACAGGAAACAAGAAAAGACTAGGG(+ACTCAC)GTACCGAGGACATTTTCTAGGAACAGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605005359_pbo8 ATCTCATCTTCTGTTCCTAGAAAATGTCCTCG(+ACTCAC)GTACCCCTAGTCTTTTCTTGTTTCCTGTGGT NGG 10 8 17 8 24 0 100 5 10