Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005445_qm8r AATAGGCCAGACCATTCTACACTTTTCCACTG(+ACTCAC)GATCCTTTCCTTATTTTTTTTGGATCATAAT NGG 10 8 17 8 24 0 100 5 10
    240605005443_cnec AACGAATTATGATCCAAAAAAAATAAGGAAAG(+ACTCAC)GATCCAGTGGAAAAGTGTAGAATGGTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605005442_il6d AGAAAGGCTTAAGAAACCCCCAGTCCCTTAAG(+ACTCAC)GTACCTAGTTTCTTACTACTACAGTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605005441_0zv9 CATGGGAGCCCACTGTAGTAGTAAGAAACTAG(+ACTCAC)GTACCTTAAGGGACTGGGGGTTTCTTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605005439_eyl9 TAGTCCCCAGGAATGAAACAATGATATGCCTG(+ACTCAC)GATCCTGCTGACCTTCGCCACCTCAACCAAT NGG 10 8 17 8 24 0 100 5 10
    240605005438_y0yh AATCAATTGGTTGAGGTGGCGAAGGTCAGCAG(+ACTCAC)GATCCAGGCATATCATTGTTTCATTCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605005437_km00 GGTGTCTTTATCAAAGGTATATATAGAATTTG(+ACTCAC)GTACCTCTCAGGTTGTATCTCTAAAGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605005435_58vy TTCCTGTAACCTTTAGAGATACAACCTGAGAG(+ACTCAC)GTACCAAATTCTATATATACCTTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605005433_ufeq CCTACACATTAAATGTTTCTTTCAGGCTGGGG(+ACTCAC)GTACCGCAAGGTGGCAGAACTTCACCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240605005432_uft2 CTATGTGCTAGGTGAAGTTCTGCCACCTTGCG(+ACTCAC)GTACCCCCAGCCTGAAAGAAACATTTAATGT NGG 10 8 17 8 24 0 100 5 10