Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005342_ac0k ATTCACAACCATCTGTAACCTGAGTTTCAGGG(+ACTCAC)GATCCAACACCTTCTTCTGGCCTCTGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605005340_6h2g CTGGTACCTACAGAGGCCAGAAGAAGGTGTTG(+ACTCAC)GATCCCCTGAAACTCAGGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605005339_j7l7 CCAGAGGGGACCTAAGACTCTAGAGCTACATG(+ACTCAC)GATCCAGGAACACTCCTAGCTTTCTTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605005337_rkuu AAGGCCCTACAAGAAAGCTAGGAGTGTTCCTG(+ACTCAC)GATCCATGTAGCTCTAGAGTCTTAGGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605005335_coux GAGTATAAACTGTGGGGTCCTCAGTCCCACAG(+ACTCAC)GATCCCAAGCCACTTGGCATGCCCAGCGAGT NGG 10 8 17 8 24 0 100 5 10
    240605005333_1767 TCATGACTCGCTGGGCATGCCAAGTGGCTTGG(+ACTCAC)GATCCTGTGGGACTGAGGACCCCACAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605005332_hpew AGTGTCTGGGTTTGATGGTTGTTTATGGGATG(+ACTCAC)GATCCAGAGGCACTTATCTGGACCTGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605005330_88kj GCCAAAGCAGCAGGTCCAGATAAGTGCCTCTG(+ACTCAC)GATCCATCCCATAAACAACCATCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605005329_x49u GACTCGTGGCTAGTACTGCTTCCCCAGACTGG(+ACTCAC)GTACCTCCTACCCCCCCTCCCCCCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240605005327_kkii GGGGTGTGTGTGGGGGGGAGGGGGGGTAGGAG(+ACTCAC)GTACCCAGTCTGGGGAAGCAGTACTAGCCAC NGG 10 8 17 8 24 0 100 5 10