Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005119_8f45 CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+ACTCAC)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240605005118_gwnt TTCCTTGGCTTCGGTTTCTGCTGCTCTCATAG(+ACTCAC)GATCCCTTAAGAGAGGGGATGACTCGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605005116_re8l TGATCCTTGACGAGTCATCCCCTCTCTTAAGG(+ACTCAC)GATCCTATGAGAGCAGCAGAAACCGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605005114_x9rt CGAGGAATCTGGTACTGGCATGCGGATTGGGG(+ACTCAC)GATCCTGAGTCCGTTTGAAAGACTGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605005113_20eg CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+ACTCAC)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240605005110_chaw TCAAATGACTGCAGCCCCAAGCACAGCCCTAG(+ACTCAC)GATCCAAACCAGGAAGAAAACAAATATTATT NGG 10 8 17 8 24 0 100 5 10
    240605005109_sl7r CCAGGAATAATATTTGTTTTCTTCCTGGTTTG(+ACTCAC)GATCCTAGGGCTGTGCTTGGGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605005108_f5ox TTGGGGGATGCCTGGGCCAGGGAGAGGCACTG(+ACTCAC)GTACCAGCCGTCCAAGCTGACAGCCTTACCC NGG 10 8 17 8 24 0 100 5 10
    240605005106_9yrp CTTAGGGGTAAGGCTGTCAGCTTGGACGGCTG(+ACTCAC)GTACCAGTGCCTCTCCCTGGCCCAGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240605005104_fwyv GCACTGAGTTCTAGGCCTCCTCTTGTCTCCTG(+ACTCAC)GATCCTCACTTGAATCCTGCAGCAGAACGGA NGG 10 8 17 8 24 0 100 5 10