Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605005031_7e29 ACACACAGACAACATGGTAGGGTTCTGAAGAG(+ACTCAC)GATCCAAGTTCCCCAGGGTAGAGTGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605005030_60qh AATCCCAGCACTTAAGGGGGTGGAGGTAAGAG(+ACTCAC)GATCCTGAGTTCAAGGCCAGCCTGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605005029_ubut TAATGTTAAGCCCAGGCTGGCCTTGAACTCAG(+ACTCAC)GATCCTCTTACCTCCACCCCCTTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605005027_p7jw GAGGCTAATACCAAACCTATAGCTACTCCTGG(+ACTCAC)GTACCCTGCCTCCAGGTGATCACAGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240605005025_vvrz CGCAAGGATGGCTGTGATCACCTGGAGGCAGG(+ACTCAC)GTACCCAGGAGTAGCTATAGGTTTGGTATTA NGG 10 8 17 8 24 0 100 5 10
    240605005023_0uo0 AGAGCACCCCATCCAACAGGCTTCTTGCCCTG(+ACTCAC)GTACCATGGAAGCACAGTTCTGGGTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240605005021_7hbb CTCAGCATGAAACCCAGAACTGTGCTTCCATG(+ACTCAC)GTACCAGGGCAAGAAGCCTGTTGGATGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605005020_5m0g CTAACTCTAAAATCCAAAAAATCTCCCAACAG(+ACTCAC)GTACCAAGAAGGTTCATTTGTAATGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605005018_d38w AAATGAACTGTCATTACAAATGAACCTTCTTG(+ACTCAC)GTACCTGTTGGGAGATTTTTTGGATTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240605005017_j0iv CTGGTATTTCAGCAACAAGTACCTTCTTGAAG(+ACTCAC)GTACCCAAAGTGATTCCAATTTTATGAAAAA NGG 10 8 17 8 24 0 100 5 10