Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004946_xbsd CATATTCCCCTAGCCTTATGAAATGCATCACG(+ACTCAC)GTACCTTAAAGTCCCCACAGGCTAGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605004945_eyl9 ATTACCAACTGCTGTCTCTCTGCTGTGAAAAG(+ACTCAC)GTACCGTAATTCACGTGATCCATCTATACGT NGG 10 8 17 8 24 0 100 5 10
    240605004944_80hr GTCACACGTATAGATGGATCACGTGAATTACG(+ACTCAC)GTACCTTTTCACAGCAGAGAGACAGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605004942_zyhc GAGAACCTAACCTTAGTTTCAGGTCAAGGTTG(+ACTCAC)GTACCTCCTGGTGGAAATTCTCATTTGGATC NGG 10 8 17 8 24 0 100 5 10
    240605004941_pk5v GAAATGATCCAAATGAGAATTTCCACCAGGAG(+ACTCAC)GTACCAACCTTGACCTGAAACTAAGGTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605004939_cnqi GGGTAATAGCCAAGTTCATATTTTTTTCCTAG(+ACTCAC)GTACCTTGTTGGGGACAATGTTGAAGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605004938_ttad TCAACCTTGACTTCAACATTGTCCCCAACAAG(+ACTCAC)GTACCTAGGAAAAAAATATGAACTTGGCTAT NGG 10 8 17 8 24 0 100 5 10
    240605004936_3x0v TAGAGGCCTGTGTTCTGGCATTAGCAAGCCAG(+ACTCAC)GTACCATGTGGATCAGAGGATTAAAAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605004935_4il6 ACACGATCTTTTTTAATCCTCTGATCCACATG(+ACTCAC)GTACCTGGCTTGCTAATGCCAGAACACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605004934_ep21 TTTTAACCACAATTTGGAAAGCTCTGTTGTTG(+ACTCAC)GTACCCAGGGATGCACTCTGTCTGTGTAGGT NGG 10 8 17 8 24 0 100 5 10