Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004914_pz9o TAAGATAAAAGGACTGAGGTCTTCCTAGCAAG(+ACTCAC)GATCCTCCCCATACCTTTGGATGTCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605004913_kq2r GCACGCTTTTCCTCCTGCTCCAAGGAGCTGAG(+ACTCAC)GATCCACCATATCCTCACCCTGACAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605004911_rl22 TGTGGCCTCCCTGTCAGGGTGAGGATATGGTG(+ACTCAC)GATCCTCAGCTCCTTGGAGCAGGAGGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605004909_zaij GATTCATGACTTCCTACAATGCCAGCTCCCAG(+ACTCAC)GATCCATTTATCTTTGCTGGAGATTACATAC NGG 10 8 17 8 24 0 100 5 10
    240605004908_0td0 CTTCTGTATGTAATCTCCAGCAAAGATAAATG(+ACTCAC)GATCCTGGGAGCTGGCATTGTAGGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240605004906_tfcd CTTCCCGGGAGCCGGTCCTCGTCAGGGCTCGG(+ACTCAC)GATCCCCCGGGACGGAGCACGTTCCCCGCAG NGG 10 8 17 8 24 0 100 5 10
    240605004903_b5ni GAGTTCTGCGGGGAACGTGCTCCGTCCCGGGG(+ACTCAC)GATCCCGAGCCCTGACGAGGACCGGCTCCCG NGG 10 8 17 8 24 0 100 5 10
    240605004901_d6j5 CATGATGCAAGCTAGAAACCTTTGTACAGGAG(+ACTCAC)GATCCCAGATGGTAGAGATGTTGGGCATATA NGG 10 8 17 8 24 0 100 5 10
    240605004900_948v GCACCTATATGCCCAACATCTCTACCATCTGG(+ACTCAC)GATCCTCCTGTACAAAGGTTTCTAGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605004858_lv18 CATAACTGTGTATTGGGAAGATGGGATGAAGG(+ACTCAC)GATCCAGTCAGAAAAGAAGAGATATCGATAA NGG 10 8 17 8 24 0 100 5 10