Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004821_mz1b CTCAGTCTGGAATTTGGAATCAACAGCTTGTG(+ACTCAC)GATCCTGAGTGTAGTAGTGTCTTACACGCGT NGG 10 8 17 8 24 0 100 5 10
    240605004820_szdg TATGGACACCTGTAGGACAGAAGAAGGTATTG(+ACTCAC)GATCCCATTACAGATGGTTGTAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605004818_j4xl ACCCCATGGTGGCTTACAACCATCTGTAATGG(+ACTCAC)GATCCAATACCTTCTTCTGTCCTACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605004816_k0gl AGTGGCCCTGGGCAGTAGCTCTTAGGGGGTGG(+ACTCAC)GTACCTCGACAGTGGATTTCAGCTTGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605004815_deur AGATGTCACTCAAGCTGAAATCCACTGTCGAG(+ACTCAC)GTACCCACCCCCTAAGAGCTACTGCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605004813_0l3u TGCTGAGATGGTGATGTTACTCTCCATTTTTG(+ACTCAC)GTACCAGTTCTGAGACTGTAAGACTTTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605004812_n26q CCCAGATGAAAAGTCTTACAGTCTCAGAACTG(+ACTCAC)GTACCAAAAATGGAGAGTAACATCACCATCT NGG 10 8 17 8 24 0 100 5 10
    240605004811_t7qt TTTTTGTGAGGGAGTTGATCCTGGGCAGTTAG(+ACTCAC)GTACCCTGGAAGGAGGGGAGGGAACCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240605004809_v6ne GCTTCATCGAGGTTCCCTCCCCTCCTTCCAGG(+ACTCAC)GTACCTAACTGCCCAGGATCAACTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605004807_4lo4 ACCGGACCTTGTGGCCAAGTAGGATTCTGAGG(+ACTCAC)GATCCAGACGAGAAGCCCAAAGAGTTCAGAT NGG 10 8 17 8 24 0 100 5 10