Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004806_5pgo CCGGCATCTGAACTCTTTGGGCTTCTCGTCTG(+ACTCAC)GATCCCTCAGAATCCTACTTGGCCACAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605004805_f34y AGTCCTTAGCAGCCCCCACCACCCACGCATCG(+ACTCAC)GATCCCATTCTCAACCAGGACCCCCGCACCT NGG 10 8 17 8 24 0 100 5 10
    240605004803_71m6 TGGACAGGTGCGGGGGTCCTGGTTGAGAATGG(+ACTCAC)GATCCGATGCGTGGGTGGTGGGGGCTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605004801_leki GTCATCTTGTCAGTTCCTAACTGGAACCTTGG(+ACTCAC)GATCCTTCTTCTTCCTCTCTTTCTTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605004759_uh3x AGAAAGAAAGAAAGAAAGAGAGGAAGAAGAAG(+ACTCAC)GATCCCAAGGTTCCAGTTAGGAACTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240605004757_m98k CTGATACTTGATGTTCCAGCCCAGCACCTCTG(+ACTCAC)GATCCTTGAAGTACCCATGATGGGTAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605004756_bwo2 AAAGGCCTATTACCCATCATGGGTACTTCAAG(+ACTCAC)GATCCAGAGGTGCTGGGCTGGAACATCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605004755_vbxd ACTCTTCCGAAGTTCTCACTCCTCCTGAACAG(+ACTCAC)GATCCCCTGACCCGTTAGGAGCCTGCTTCGT NGG 10 8 17 8 24 0 100 5 10
    240605004753_ndj5 TGTGCACGAAGCAGGCTCCTAACGGGTCAGGG(+ACTCAC)GATCCTGTTCAGGAGGAGTGAGAACTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240605004752_39ea TCCTTGCTCACCCAAACTAGATATATATTTGG(+ACTCAC)GATCCATGGTTGTTCAGACTTTACATGAAGA NGG 10 8 17 8 24 0 100 5 10