Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004659_31oz CATGCCAAGAACAGCCCCCTGACTTCCACTGG(+ACTCAC)GATCCAGGACAGATACTGTCTGTCCTAACCC NGG 10 8 17 8 24 0 100 5 10
    240605004658_m0oi CCTCAGTAACCCTGCATGTGTAATGGGCTGTG(+ACTCAC)GTACCATCACACTGCCCATGTGCCGTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605004657_njxk AGTCAGAGCCACGGCACATGGGCAGTGTGATG(+ACTCAC)GTACCACAGCCCATTACACATGCAGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605004655_qpc7 CAATTATTTGGTGTGCCCTTTCCACATAAGTG(+ACTCAC)GTACCCAATCTAGACAAACCACAGGCTACAC NGG 10 8 17 8 24 0 100 5 10
    240605004653_854a GATGTGTGTAGCCTGTGGTTTGTCTAGATTGG(+ACTCAC)GTACCACTTATGTGGAAAGGGCACACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605004652_60r0 TTTAGACAAAAGTTGGTGCTGCTGTGGCGCAG(+ACTCAC)GATCCTGGACTGTGGGGAGGGGAGGAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605004651_ybi7 ACCCCAGGGCTCCTCCCCTCCCCACAGTCCAG(+ACTCAC)GATCCTGCGCCACAGCAGCACCAACTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605004649_cbmb ATAAAAATTATACACGAGACCTGCTGTTTTGG(+ACTCAC)GTACCTGGCTACTGCCCAGAGTGCAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240605004647_ts4v ATGTTTCTCATTGCACTCTGGGCAGTAGCCAG(+ACTCAC)GTACCCAAAACAGCAGGTCTCGTGTATAATT NGG 10 8 17 8 24 0 100 5 10
    240605004646_k4c2 CTCAGCAAGCTGGGATGTAGTGAAGCCTCGAG(+ACTCAC)GATCCCCACTGTCAGAGGCTCTCTTTTGGTT NGG 10 8 17 8 24 0 100 5 10