Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004734_m0c3 TATAGAATGTTAAAGGACTTACCTTACAAATG(+ACTCAC)GTACCTAACTACCTTATAAATACGCACATTG NGG 10 8 17 8 24 0 100 5 10
    240605004733_ys0d GAACTCTCTATTTTCCAGAATGCTTTGTCAAG(+ACTCAC)GATCCTCTGTGGTCTGTGGCAGTTTTATCAG NGG 10 8 17 8 24 0 100 5 10
    240605004732_k7uf GCTTCCTGATAAAACTGCCACAGACCACAGAG(+ACTCAC)GATCCTTGACAAAGCATTCTGGAAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240605004730_58jl AGGACAGAAGAGGAGAAAGGGAAGGCCTGAGG(+ACTCAC)GTACCTCAGCCGCCAGCTACCTTCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605004729_w95q AGACTTCAAAGTGAAGGTAGCTGGCGGCTGAG(+ACTCAC)GTACCCTCAGGCCTTCCCTTTCTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605004727_f2gx GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+ACTCAC)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240605004725_qsou GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+ACTCAC)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605004723_1pmm TTATGCAGATGCTCTTGCTGTGTTCCTCAAAG(+ACTCAC)GATCCAAGCCATAAAATTCTTTATGCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605004722_3wwx ACTGTGACATGCATAAAGAATTTTATGGCTTG(+ACTCAC)GATCCTTTGAGGAACACAGCAAGAGCATCTG NGG 10 8 17 8 24 0 100 5 10
    240605004721_vuwy AATGAAGTTCACTGTGTTTATAAACTGAAAAG(+ACTCAC)GTACCGGGTCTTACAGCATTTTATATATCAC NGG 10 8 17 8 24 0 100 5 10