Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004644_f440 TCCTGAACCAAAAGAGAGCCTCTGACAGTGGG(+ACTCAC)GATCCTCGAGGCTTCACTACATCCCAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605004642_0yfk CTGCCCCAGATTCCTCCCCATCACCTCTCTGG(+ACTCAC)GTACCTGATTTCCTGGTCTGTGCACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605004641_om8c CCAAGGCTGCAGTGCACAGACCAGGAAATCAG(+ACTCAC)GTACCCAGAGAGGTGATGGGGAGGAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605004639_tbsk TGACTCCCCAGTGGAGGCAAGCCAGAGGTCTG(+ACTCAC)GATCCTCCACCCTCTCCTGGAGTGCCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605004638_2muk CTTACTCTGGGGCACTCCAGGAGAGGGTGGAG(+ACTCAC)GATCCAGACCTCTGGCTTGCCTCCACTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605004636_00wv GGGGAGAAGGCCTGGCATCAAAAAGCTGCCAG(+ACTCAC)GATCCGCGACAAGGGGACGGACAAAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240605004635_7ki0 AGGGCTCTCATTTTGTCCGTCCCCTTGTCGCG(+ACTCAC)GATCCTGGCAGCTTTTTGATGCCAGGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605004633_l863 CAGCACAAGATGACTGGCCACTACCGGGGTGG(+ACTCAC)GTACCGCAGGGTTTGGGTTCAGGCTCTACAC NGG 10 8 17 8 24 0 100 5 10
    240605004631_jmpd CTGTTGTGTAGAGCCTGAACCCAAACCCTGCG(+ACTCAC)GTACCCACCCCGGTAGTGGCCAGTCATCTTG NGG 10 8 17 8 24 0 100 5 10
    240605004630_bqdd ATTCATGCTGGCAATTGAGCGCATAATCGCTG(+ACTCAC)GTACCATGAACATGATATAACTTTTACTAAG NGG 10 8 17 8 24 0 100 5 10