Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004543_5rr4 AAAACCCCTTTATTCTTGTTGCTTTTACAGTG(+ACTCAC)GTACCGTGCATGCGAACCAGGAGAATTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605004542_cpja GGAGGAGAGGTGGCGCCCAGCAGGTGGGTGAG(+ACTCAC)GATCCCCAGTGGTTGGCAGGGTGCCTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605004540_lfmg CCGCAAAGCCAGGCACCCTGCCAACCACTGGG(+ACTCAC)GATCCTCACCCACCTGCTGGGCGCCACCTCT NGG 10 8 17 8 24 0 100 5 10
    240605004538_7hbw CATATATGCCTGAAAGCCAGAAGAGGGCACCG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605004536_r1fi ACCACATGGTGACTCACAACCATCTGTAATGG(+ACTCAC)GATCCGGTGCCCTCTTCTGGCTTTCAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605004535_c0ws TGCAACTATATTTTTAAAAAGTACAAAACTAG(+ACTCAC)GATCCTTACCTTCTGAATTAAAAATACAATT NGG 10 8 17 8 24 0 100 5 10
    240605004534_2iph TGATGAATTGTATTTTTAATTCAGAAGGTAAG(+ACTCAC)GATCCTAGTTTTGTACTTTTTAAAAATATAG NGG 10 8 17 8 24 0 100 5 10
    240605004532_3w7a ACCACATGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCGATATCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605004531_enjs ACTGTCTTCAGACACACCAGAAGAGGATATCG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605004530_tkrt GAGAGGTGGCCTGGGACTCTGTCCTCCATATG(+ACTCAC)GTACCTCAGGAATGCACATCCCTACGATACA NGG 10 8 17 8 24 0 100 5 10