Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004454_06ev ATTATATGCATTAACACAGCTCTGTAAAGTAG(+ACTCAC)GTACCATTGTACTTTTCATAGGAGGAAACAG NGG 10 8 17 8 24 0 100 5 10
    240605004453_84g4 CCTTGCACAAAATGGAGTTTCTCATCCTGCTG(+ACTCAC)GATCCTTTAATGTGTCAGTCCCTGGAAGACC NGG 10 8 17 8 24 0 100 5 10
    240605004452_h02t GTACAGGTCTTCCAGGGACTGACACATTAAAG(+ACTCAC)GATCCAGCAGGATGAGAAACTCCATTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605004450_ixxa TCTCCTGGGCATACCCCAGGGAGGAAAGTAAG(+ACTCAC)GTACCCATGGCCAGCCCTGGCTCCTAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605004448_yudf AGGATGGCATTAGGAGCCAGGGCTGGCCATGG(+ACTCAC)GTACCTTACTTTCCTCCCTGGGGTATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605004446_46rj GAAGGGAAGCTCTGCCCACCCTCAAGTCCCTG(+ACTCAC)GATCCTGAGCCAGAGTCAATTCTATACTGTA NGG 10 8 17 8 24 0 100 5 10
    240605004444_hjo8 GTGACTACAGTATAGAATTGACTCTGGCTCAG(+ACTCAC)GATCCAGGGACTTGAGGGTGGGCAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605004443_05r8 CAAGGCAGCACGCTCAGAGGACCTGGAGCAGG(+ACTCAC)GATCCCGCAGTAAAGCAGCAGCAGATGTCTA NGG 10 8 17 8 24 0 100 5 10
    240605004441_bao6 GTTTGTAGACATCTGCTGCTGCTTTACTGCGG(+ACTCAC)GATCCCTGCTCCAGGTCCTCTGAGCGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605004440_a9rk CTTCCTCCTTGAAAGCAGGGTTACCCTTAAAG(+ACTCAC)GATCCCTGGAGCCCCTCCCATAAGAGGCCCT NGG 10 8 17 8 24 0 100 5 10