Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004346_kka5 ATAATCCTAGCAATGGGGAGCAGAAATAGGAG(+ACTCAC)GATCCCTGGGGAATAGGGCAATCCCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605004345_9sro AAAAGACCTCAGGCACCTGAGCACTTTGACAG(+ACTCAC)GTACCAGGTAGAGTCCATCCTTGCATCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605004343_i9kp AGGAACACTGATGCAAGGATGGACTCTACCTG(+ACTCAC)GTACCTGTCAAAGTGCTCAGGTGCCTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605004341_pgak GCAGGTCCCACGGTGTTTGGAGATGGCTTGGG(+ACTCAC)GATCCGTGAGGATGGCATGACCAGCTAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605004339_c1kg TCTCGGGGCTAGCTGGTCATGCCATCCTCACG(+ACTCAC)GATCCCCAAGCCATCTCCAAACACCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605004338_pote CCCCTTTCATGGTCCGTTTTCTTTTCAAACTG(+ACTCAC)GATCCAAAAAGAGACGAGGGTTTTCTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605004337_5zjn CCTAGAGGAAAGAAAACCCTCGTCTCTTTTTG(+ACTCAC)GATCCAGTTTGAAAAGAAAACGGACCATGAA NGG 10 8 17 8 24 0 100 5 10
    240605004336_kuw7 CTGGTAAAACTACTGCAGTGGGGCTTTGACAG(+ACTCAC)GATCCAAGTTATCACCAGAAAACAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240605004334_sozf CACTTTTTGTTTTGTTTTCTGGTGATAACTTG(+ACTCAC)GATCCTGTCAAAGCCCCACTGCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605004333_a9d2 CCAAATACAGAAAAGAGGAGGCCTGAAAGAGG(+ACTCAC)GTACCAGGGGACAGGGAGCCATGGGGGCCTA NGG 10 8 17 8 24 0 100 5 10