Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004438_cw18 TCAACAGGGCCTCTTATGGGAGGGGCTCCAGG(+ACTCAC)GATCCTTTAAGGGTAACCCTGCTTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605004436_sh1a GGTTGAGTTCTGGAGCAGTTGCAGGTAGCACG(+ACTCAC)GTACCCCGGAAATGGGAAGTCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605004434_l8gz TGAGGACTGTCCAGAGACTTCCCATTTCCGGG(+ACTCAC)GTACCGTGCTACCTGCAACTGCTCCAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605004432_txhu ATCTTCCCTCGGCTGATCCAGCAGCCTGGGTG(+ACTCAC)GTACCGGTGTGCATTGCCGTATCCAGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605004431_77cv GAGTACAAGACTGGATACGGCAATGCACACCG(+ACTCAC)GTACCACCCAGGCTGCTGGATCAGCCGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605004429_6lrc AATACTGACAGTTAATATGATGGGCCACTAGG(+ACTCAC)GATCCCAGAAAGTTAAGAGACATTTCTAACT NGG 10 8 17 8 24 0 100 5 10
    240605004427_hq5h ATAGTAGTTAGAAATGTCTCTTAACTTTCTGG(+ACTCAC)GATCCCTAGTGGCCCATCATATTAACTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605004425_n6fl GTAACTACTTTCTCCCCCAAAAGTGTCTAGGG(+ACTCAC)GATCCAGTTTGCCTTTGTTAGAAGACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240605004424_084i TACAGTTGCAGTCTTCTAACAAAGGCAAACTG(+ACTCAC)GATCCCCTAGACACTTTTGGGGGAGAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605004422_rrk3 CCGGCATTTCAGCGTCAAGTGCACTTAGCGGG(+ACTCAC)GTACCCAGCCCCCCCAGTCCCAACACCTGTC NGG 10 8 17 8 24 0 100 5 10