Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004330_41or AACCCTAGGCCCCCATGGCTCCCTGTCCCCTG(+ACTCAC)GTACCCTCTTTCAGGCCTCCTCTTTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605004328_4bjp ATTCCCAGTCAGCTTCAGGGGAATGAGAAAGG(+ACTCAC)GATCCTACTAATGTGGAAAGAAGCAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240605004327_xrnj GGAGGGATGCTTGCTTCTTTCCACATTAGTAG(+ACTCAC)GATCCCTTTCTCATTCCCCTGAAGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240605004325_84mc CCTTGCTGTTAATTTTGCATATGCTAAAGCGG(+ACTCAC)GATCCCCTGGAGGCGGCATTTTCCCTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605004322_go3x TGCAGAGCTAAGGGAAAATGCCGCCTCCAGGG(+ACTCAC)GATCCCGCTTTAGCATATGCAAAATTAACAG NGG 10 8 17 8 24 0 100 5 10
    240605004320_l27i GAGTTTGCAGGTGGGTTTTGAGGGTGTCCATG(+ACTCAC)GATCCCTGTCTGGAAGCTTGAGTCTTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605004319_edur CCAGTTACCAAAGACTCAAGCTTCCAGACAGG(+ACTCAC)GATCCATGGACACCCTCAAAACCCACCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605004317_oxyp CACTCCCCTAGTAGAGATTTGGTCTCTAACAG(+ACTCAC)GTACCCTGGCAATCATGGGCTTGGATCACTA NGG 10 8 17 8 24 0 100 5 10
    240605004315_445c GTGTGTAGTGATCCAAGCCCATGATTGCCAGG(+ACTCAC)GTACCTGTTAGAGACCAAATCTCTACTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605004313_jaz2 CCAATGCTCAGGAACATGGGGAAAGGGCTGTG(+ACTCAC)GATCCCTGCTGTGAAGTGGCAGGTCTGACTT NGG 10 8 17 8 24 0 100 5 10