Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004511_o477 CTCTTGGATGAGTCTCAGGAGAACAGATCTGG(+ACTCAC)GTACCAGGACTAAGGCAAGATCCTGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605004510_lp5o CCAGCCTCCCATAAGGGTCAATACAACGCCTG(+ACTCAC)GATCCCCAAACCTGTGCTCAGGAGGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605004508_9lyl AGCCCCAGCTTCCTCCTGAGCACAGGTTTGGG(+ACTCAC)GATCCAGGCGTTGTATTGACCCTTATGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605004506_eahh CCCTGATGGAGGAGGCCAGGGCTCAGAGCAGG(+ACTCAC)GTACCCACTGTGGATCTTTGTCTGTGAACCG NGG 10 8 17 8 24 0 100 5 10
    240605004504_9eiy GTTGTCGGTTCACAGACAAAGATCCACAGTGG(+ACTCAC)GTACCCTGCTCTGAGCCCTGGCCTCCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605004501_rzlj TCTCGAGACTGACTCAGGGTCCAGAGCCACCG(+ACTCAC)GATCCATCCCCAAGTGGAGATGATGTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240605004500_sklu AACCTCTCCGACATCATCTCCACTTGGGGATG(+ACTCAC)GATCCGGTGGCTCTGGACCCTGAGTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605004458_vdj7 CAAGTTCGAGCCAGATATGGAGATCAGGAAGG(+ACTCAC)GTACCTTCTGAGGCTTGTAGGCATGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605004457_9pcz GCTTGCTCCTCCATGCCTACAAGCCTCAGAAG(+ACTCAC)GTACCCTTCCTGATCTCCATATCTGGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605004456_vxye GAAGCCTGTTTCCTCCTATGAAAAGTACAATG(+ACTCAC)GTACCTACTTTACAGAGCTGTGTTAATGCAT NGG 10 8 17 8 24 0 100 5 10