Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605004838_o57a GGAGTGTGCATGATAATGCCAAAGCTGCCCAG(+ACTCAC)GATCCCTGCAAAATAACAGAGCAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240605004836_0nqb GCAACCCAATAATACTATCATAATCCGGACAG(+ACTCAC)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605004835_e2f1 TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+ACTCAC)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240605004833_l8y6 TTGGTAGTTCATTCGAAAGTTGAGCTCATCCG(+ACTCAC)GTACCGGGCTGGTTTCCTATGAATGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605004830_o5xr CTCAGTGAACTCATTCATAGGAAACCAGCCCG(+ACTCAC)GTACCGGATGAGCTCAACTTTCGAATGAACT NGG 10 8 17 8 24 0 100 5 10
    240605004829_755j GGGGCCCCCTGCCCGCGCCCAGACACTTGATG(+ACTCAC)GATCCCCACCAACGCCCCCCCTACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605004827_ksxr GAGGTGGTGGGGGTAGGGGGGGCGTTGGTGGG(+ACTCAC)GATCCATCAAGTGTCTGGGCGCGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605004826_yqfb CTCTGGACCACTGAGCCATCTCCAACTTCTTG(+ACTCAC)GATCCTTGGGACAACAAAAGGAGAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605004823_9qus AGAGTGGCAGCCTCTCCTTTTGTTGTCCCAAG(+ACTCAC)GATCCAAGAAGTTGGAGATGGCTCAGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605004822_7xnj AGCGAACGCGTGTAAGACACTACTACACTCAG(+ACTCAC)GATCCACAAGCTGTTGATTCCAAATTCCAGA NGG 10 8 17 8 24 0 100 5 10